Capillary Isoelectric Focusing
نویسنده
چکیده
Beale SC (1998) Capillary electrophoresis. Analytical Chemistry 70: 279. (This journal prints every two years a review on capillary electrophoresis. The article is written by an expert in the Reld and covers more or less all developments in CE including CGE of the preceding years.) Grossmann PD and Colburn JC (eds) (1996) Capillary Electrophoresis: Theory and Practice. San Diego: Academic Press. Grossmann PD and Soane DS (1991) Capillary electrophoresis of DNA in entangled polymer solutions. Journal of Chromatography 559: 257. This paper treats the two models for separation in CGE (Ogston and reptation) in more detail. JoK rgenson JW and Lukacs KD (1981) High resolution separation based on electrophoresis and electro-osmosis. Analytical Chemistry 53: 1298. Kuhn R and Hoffstetter-Kuhn S (1993) Capillary Electrophoresis: Principles and Practice. New York: Springer Verlag. Landers JP (ed.) (1994) Handbook of Capillary Electrophoresis. Boca Raton: CRC. Li SFY (1992) Capillary Electrophoresis. Amsterdam: Elsevier. Lunte SM and Radzik DM (1996) Pharmaceutical and Biomedical Applications of Capillary Electrophoresis. Oxford: Pergamon Press. Schwartz H and Guttman A (1995) Separation of DNA by Capillary Electrophoresis. Fullerton, CA: Beckmann Primer 607397.
منابع مشابه
Whole-Column-Imaging Detection for Capillary Isoelectric Focusing and Capillary Electrophoresis
Despite the significant advantages of capillary isoelectric focusing over gel isoelectric focusing, widespread acceptance of the technique as a replacement for gel isoelectric focusing has not occurred. Several factors contribute to capillary isoelectric focusing’s slower-than-expected adoption; perhaps the most important are the performance and procedural difficulties introduced when capillary...
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Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum albumin, beta-lactoglobulin, horse myoglobin, cytochrome c) has been chosen as a peptide test mixture. After assessment of different modes of ...
متن کاملAnalysis of -Keratins in the Horns of Rhinoceros and Buffalo by Non-Native Capillary Isoelectric Focusing
A capillary isoelectric focusing (CIEF) method under denaturing condition has been developed for separation of a-keratins, highly cross-linked biomacromolecules, in horns of rhinoceros and buffalo. The a-keratins were denatured in 8 M urea and separated in the presence of ampholytes with applicable pH ranges of 3-7 or 3-10. In the preliminary results, it was demonstrated that the o~-keratins in...
متن کاملIsoelectric focusing sample injection for capillary zone electrophoresis in a fused silica capillary.
An improvement has been made to couple isoelectric focusing (IEF) sample injection and capillary zone electrophoresis in an untreated fused silica capillary. Electroosmotic flow is efficiently prevented by simply using a rubber block at the outlet end of the capillary during IEF sample injection. The experimental conditions that affect the concentration effect are discussed. A concentration enh...
متن کاملSimple and Highly-sensitive Enzyme Activity Assay Based on Reagent-release Capillary - Isoelectric Focusing (rrc-ief) towards the Development of Multi Analyte Sensing Micro Device Capable of Detecting Both Proteins and Enzyme Activities
We developed a simple and highly-sensitive enzyme activity assay method based on reagent release capillary – isoelectric focusing (RRC-IEF), that can be applied for the development of multi analyte sensing device allowing simple and highly sensitive multi assay of both proteins and enzyme activities.
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